Preliminary indentification of mungbean breeding accessions for resistance to bruchids (Callosobruchus chinensis and C. maculatus) using RAPD markers
1996
Cheng Xuzhen | Peerasak Srinives | Charles Y. Yang (Chinese Academy of Agricultural Sciences. Institute of Crop Germplasm Resources)
Random amplified polymorphic DNA (RAPD) markers were used to detect mungbean breeding accessions for resistance to bruchids. Polymerase chain reaction (PCR) conditions suitable for discriminating mungbean bruchid resistant genotypes were determined. Primers that produced consistent and repeatable bands between the parents and different cross combinations were chosen to conduct RAPD analysis. The results showed that 5 selected primers can be used for identifying sixteen mungbean accessions. They could be classified into 4 groups, i.e. wild type resistance group, cultivar resistance group, progeny resistance group, and susceptible varieties and their progenies group. Moreover, the last group could be classified into 6 subgroups and families, viz. V2802 resistance subgroup, VC3890A family, VC1973A family, VC1178A family, VC2778A family, and CN60 subgroup.
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This bibliographic record has been provided by Thai National AGRIS Centre, Kasetsart University
