Effect of osmotic pressure on sperm motility of Mekong giant catfish and Chao Phraya catfish
1996
Krit Mongkonpunya | Thusanee Pupipat | Anong Hambananda (Kasetsart Univ., Bangkok (Thailand). Faculty of Science. General Science Dept.)
Motility of sperm cells of the catfishes pangasius gigas and Pangasius hypophthalmus was estimated over a range of osmotic pressures. The osmotic pressure of threshold activation (25 percent motile sperm cells in a sample) was 240 +- 10 mOsm/kg for the sperm of P. gigas, and 221 +- 12 mOsm/kg for P. hypophthalmus. The osmotic pressure of complete activation (highest percentage of motile cells observed in a sample) was 186 +- 9 mOsm/kg for the sperm of P. gigas, and 98 +- 21 mOsm/kg for P. hypophthalmus. These experiments were performed using dilutions of calcium-free Hanks' balanced salt solution (C-F HBSS) which is highly ionic. Similar experiments were performed using diluted glucose solutions to evaluate the activation of sperm in ion-deficient solutions. The osmotic pressure in glucose solutions of threshold activation was 248 +- 27 mOsm/kg for sperm of P. gigas, and 209 +- 16 mOsm/kg for P. hypophthalmus; the osmotic pressure of complete activation was 187 +- 12 mOsm/kg for sperm of P. gigas, and 98 +- 24 mOsm/kg for P. hypophthalmus. These values were not significantly different from those obtained using diluted C-F HBSS. The role of particular ions in the activation of sperm of these species is unknown. Reduction in osmotic pressure appears to play a major role in activation, with the sperm of P. gigas being more sensitive to reduction of osmotic pressure than the sperm of P. hypophthalmus.
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