Establishment of experimental conditions on random amplified polymorphic DNA (RAPD) analysis of Lansium domesticum Corr. I. DNA extraction from leaf samples
2000
Charassri Nualsri | Suvimon Konlasuk (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Plant Science)
DNA extraction from leaf samples of longkong (Lansium domesticum Corr.) was investigated. From four different methods, the CTAB method modified from Doyle and Doyle (1990) showed the best result. From 200 mg fresh leaves as starting material, approximately 15-20 micro g of DNA was obtained. DNA extracted from leaves in three different stages of development was compared. Young leaves tended to produce higher yields while young fully expanded leaves and old leaves gave almost the same amounts of DNA. Quantity and quality of extracted DNA from leaves that were kept in a sealed brown paper bag and stored in various durations under 3 different conditions, room temperature (30-32 deg C), 4 deg C and -30 deg C, were determined. At room temperature and 4 deg C, DNA yields declined rapidly after storage for 3 and 5 days, respectively, while leaves storage under -30 deg C could be kept at least 30 days and the banding profiles of RAPD-PCR products was not different from fresh leaf extracted DNA.
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