Embryo transfer between Murrah buffalo and Thai swamp buffalo
1993
Gunjiro, Kobayashi | Mongkol Techakumphu | Peerasak Chantaraprateep | Chainarong Lohachit (Chulalongkorn Univ. Bangkok (Thailand). Faculty of Veterinary Science. Dept. of Obstetrics Gynaecology and Reproduction)
Feasibility of embryo transfer from Murrah buffalo to Thai swamp buffalo was studied. Embryos were collected by nonsurgical method from five Murrah buffaloes either after single ovulation or after superovulation. From single egg collection, 80 percent (4/5) of recovery rate and 75 percent (3/4) of normality rate were obtained. While with the superovulation by PMSG at the dose of 2400-2800 IU, the average ovarian response in term of corpus lutea and follicles with diameter more than 5 mm was 11.0 +- 3.8 and the ovulation was 4.4 +- 4.3. The percentage of recovered eggs was 45.5 percent (10/22). Eight normal embryos (1/8 from single egg collection and 7/8 from superovulation) were transferred to seven recipients. Pregnancy diagnosis was performed by progesterone assay and rectal palpation at 60-90 days after the transfer. No pregnancy was found, but four from seven recipients had a high level of progesterone at Day 21 of the estrus cycle. This showed that Murrah buffalo embryo can survive in uterus of swamp buffalo recipient but the development cannot continue until term.
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