Detection of citrus greening bacterium by immunoblotting
1994
Ratana Sdoodee | Garnett, H. (Prince of Songkla Univ., Songkhla (Thailand). Faculty of Natural Resources. Dept. of Pest Management)
Polyclonal antibodies raised against cultured African greening bacterium were successfully used in immunoblotting assays for detection of Thai greening. The assays were made in crude sap of citrus midribs collected from suspected diseased trees throughout Thailand. The results of immunoblotting tests indicated that the antisera were specific to Thai greening, as 130/190 Som Khieo Waan (Siam mandarin), 21/23 Shogun (Shogun mandarin), 30/139 Som Chuk (Neck orange), 12/27 pomeloes, and 9/9 Som Cheet (calamondin) gave positive reactions, while non of the healthy controls did. The incidence of greening in the tested trees showing reactions with greening antsera was confirmed by electron microscopy indicating the presence of pleomorphic greening bacterium-like organisms in their phloem cells. Transmission of putative greening from positive citrus trees by insect vector (Diaphorina citri Kuwayama) to healthy index plants was attempted to support results from immunoblotting assays. The greenings were transmitted from 4/4 Som Chuk and 6/6 Shogun.
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