Preliminary study of detection for plant viral diseases by ELISA
1998
U-thai Rungruangsi, Maejo Univ., Chiang Mai (Thailand). Biotechnology Centre
ELISA (enzyme-linked immunosorbent assay) has been in general use for detecting plant viruses. In Thailand, cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV) are found in a wide variety of economic crops and thus, deserve more attention especially when mixed infection occurs. The present study reports a workable sanwich-mode ELISA protocol using commercial antiviral antibodies form the U.S. The developed protocol led to a comparative study with the more time-consuming bioassay which was aimed to detect CMV-TMV mixed infection. It was concluded that both methods should be utilized as they complement each other.
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This bibliographic record has been provided by Thai National AGRIS Centre, Kasetsart University