Development of random amplified polymorphic DNA (RAPD) technique for indentification of pepper
1999
Thirachai Thananan (Thammasat Univ. The Rangsit Center, Pathum Thani (Thailand). Faculty of Science and Technology. Department of Biotechnology)
Pepper is one of the most important vegetable in a way of daily Thai life. I was applied random amplified polymorphic DNA (RAPD) technique for identity of the pepper cultivars. Fourteen cultivars of pomelo have been collected. 72 random primers were screened and 70 primers or about 97 percent of them responded with the pepper DNA. 20 primers which gave clear amplified products were used to analyze all the DNA samples. The result showed significant differences among the 14 cultivars. All cultivars could be identified by using one primer. However, using more primers and simultaneously compared with the standard cultivars should be done for a reliable result. A comparison of RAPD patterns from each cultivar with another can analyzed evolutionary relationship.
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