Comparative study of ethylenediaminetetraacetic acid and nitric acid as bone decalcification agents in ostrich bones
2004
Pittaya Papirom(Khon Kaen University, Khon Kaen (Thailand). Faculty of Veterinary Medicine. Dept. of Pathobiology) | Suthida Viriyametharoj(Khon Kaen University, Khon Kaen (Thailand). Faculty of Veterinary Medicine. Dept. of Pathobiology) | Suttisak Nopwinyoowong(Khon Kaen University, Khon Kaen (Thailand). Faculty of Veterinary Medicine. Dept. of Pathobiology) | Nusra Suwannachot(Khon Kaen University, Khon Kaen (Thailand). Faculty of Veterinary Medicine. Dept. of Pathobiology)
Comparison study of ostrich bone decalcified solution between 5 percent and 10 percent (w/v) of ethylenediaminetetraacetic acid (EDTA) and nitric acid was performed. The result showed that 10 percent (w/v) EDTA completely decalcified the ostrich bone on day 15 and has no effect on staining, whereas 5 percent (w/v) EDTA could not decalcify the ostrich bone. Both 5 percent and 10 percent (w/v) nitric acid completely decalcified the bone on day 9 as shown by an over eosin staining with hematoxylin and eosin stain. The over eosin staining of tissue may be caused by nitric acid.
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