Stomatal size, stomatal frequency and pollen grain diameter as indirect method for identification of ploidy levels in cotton
2005
Arunee Wongpiyasatid(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Applied Radiation and Isotopes) | Praparat Hormchan(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Entomology) | Katarut Chusreeaeom(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Science. Department of Applied Radiation and Isotopes) | Ngamchuen Ratanadilok(Kasetsart University. Bangkhen Campus, Bangkok (Thailand). Faculty of Agriculture. Department of Agronomy)
Seeds of diploid local variety cotton (Gossypium arboreum), PM2, were treated with 0, 0.1 and 0.5 percent colchicine solutions at duration of 24 and 36 hours for polyploidy induction. Seed germination, seedling survival, stomatal size, stomatal frequency, percent pollen grain sterility and pollen grain diameter were recorded. The results showed seed germination, seedling survival and stomatal frequency to be negatively correlated with colchicine concentrations and durations of seed soaking while numbers of plant having large stomatal size and percent pollen grain sterility have positive correlation. Stomatal size increased whereas stomatal frequency decreased. No correlation between pollen grain diameter and colchicine concentrations as well as durations of seed soaking was found.
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This bibliographic record has been provided by Thai National AGRIS Centre, Kasetsart University
