Gross and histopathological changes of Pacific white shrimp (Litopenaeus vannamei) experimentally induced with contaminated of melamine plus cyanuric acid in pelleted feed | การศึกษาลักษณะภายนอกและการเปลี่ยนแปลงทางพยาธิสภาพของเนื้อเยื่อในกุ้งขาวแวนนาไมที่ได้รับอาหารเม็ดสำเร็จรูปผลิตจากวัตถุดิบที่ปนเปื้อนของเมลามีนและกรดไซยานูริค
2009
Chalor Limsuwan(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) | Niti Chuchird(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) E-mail:[email protected] | Satit Prasertsri(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) | Sutee Wongmaneeprateep(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) | Kesinee Laisutisan(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) | Pattama Wiriyapattanasub(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center) | Kaewta Limhang(Kasetsart University, Bangkok (Thailand). Faculty of Fisheries. Aquaculture Business Research Center)
This studyaim to determine the effects of melamine and cyanuric acid contaminated feed on theclinical sign and histopathological changes of Litopenaeus vannamei.Sixty juvenile white shrimp weighing 2 g were fed four times daily with pelleted feed contained 100-120 mg melamine and 80-100 mg cyanuric acid per kg of feed and rearing in seawater at salinity 25 ppt for 70 days. Water temperature was maintained consistently at 28+-1 deg C. Some shrimp developed swollen cephalothorax after 30 days, which became more prominent at day 70. After removing the carapace, a medium to large watery sac was observed. Some shrimp had whitish spots or patches on the hepatopancreas. Fresh squashed whitish spots revealed the presence of crystalline precipitate under a light microscope. Histopathological changes were observed from both shrimp groupswith swollen cephalothorax and whitish spots. Irregular shape of crystals wasobserved in antennal glands, and diffuse necrotic cells associated with crystals were found in coelomosac complex. Lymphoid organ spheroids were found from affected shrimp but without crystal precipitate as well as hepatopancreas and hematopoietic tissue. This results indicate that shrimp feed containing melamine and cyanuric acid could produce insoluble crystals in the antennal glands that obstructed and impaired theexcretory system to cause swollen cephalothorax.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Kasetsart University