Screening of probiotic lactic acid bacteria from piglet faeces
2010
Buasai Petsuriyawong(King Mongkut's University of Technology Thonburi, Bangkok (Thailand). Faculty of Science. Department of Microbiology) E-mail:buasaip@hotmail.com | Nongpanga Khunajakr(King Mongkut's University of Technology Thonburi, Bangkok (Thailand). Faculty of Science. Department of Microbiology)
Aim of this research was to screen for potential probiotic lactic acid bacteria (LAB) from piglet faeces. A total of 317 LAB were isolated from 136 samples of pig faeces. Preliminary screening was based on antimicrobial activity against 8 pathogenic bacteria. One hundred seventy one isolates, which exhibited antimicrobial activity against at least three of indicator strains, were selected and tested for acid and bile tolerance, bile salt hydrolase (BSH) activity and antibiotic susceptibility. Only five isolates, one identified as Lactobacillus amylovorus and four as Lactobacillus reuteri by sequence analysis of 16s rRNA showed high survival under simulated gastric and intestinal conditions, and were selected for further characterization. These five selected isolates also exhibited bile salt hydrolase activity. All five isolates were resistant to penicillin G, oxytetracyclin, neomycin, lincomycin and vancomycin, but sensitive to rifampicin and bacitracin. Based on their probiotic properties, one strain of L. amylovorus and four strains L. reuteri were considered as potential candidate probiotic for animal feed.
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This bibliographic record has been provided by Thai National AGRIS Centre, Kasetsart University
