Production and characterization of monoclonal antibodies specific to GLRa-2, GLRaV-6 and GVD
1999
Zhong, Z.
Monoclonal antibodies specific to GLRaV-2 (18), GLRaV-6 (3), and GVD (6) were produced to improve the immunoenzymatic detection of these viruses. Six out 18 Mabs specific for GLRaV-2 proved to the elicited by surface epitopes. The other twelve were evidently elicited by criptotopes, because did not decorate virus particles by IEM, but could detected virus coat protein in western blot. Interestingly, when virus were detected by TAS-ELISA, using extracts of mature canes, all six surface epitope specific Mabs were more sensitive than critotope specific Mabs. In a survey carried out in Apulia region, GLRaV-6, appeared to be heavily spread in cvs. Early-Cardinal and Cardinal no GLRaV-6 pure source were identified. Six specific GVD-Mabs were selected, whose use in TAS-ELISA allowed to detect of GVD in both Nicotiana and grapevine tissues. Serological relationship between GVA and GVD, were confirmed.
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This bibliographic record has been provided by International Center for Advanced Mediterranean Agronomic Studies - Mediterranean Agronomic Institute of Montpellier
