Application of solid-phase microextraction in cocoa volatiles analysis
2006
Suzannah Sharif | Aznie Aida Ahmad, Malaysian Cocoa Board, Lot 3, Jalan P/9B, Seksyen 13, 43650 Bandar Baru Bangi, Selangor (Malaysia), Chemistry and Technology Division
Solid phase micro extraction (SPME) is a sample preparation technique using a fused-silica fiber that is coated on the outside with an appropriate stationary phase. Analytes, which can be in the headspace or in liquid, is directly extracted to the fiber coating. The SPME technique can be used routinely in combination with gas chromatography (GC), GC-mass spectrometry (GC-MS), high performance liquid chromatography (HPLC) or LC-MS. This technique had been used in environmental analysis, aroma and flavor analysis of foods. For the analysis of cocoa volatiles in roasted nib and cocoa powder, the optimum condition was 15 mins stabilization at 60°C and extraction for 15 mins. Comparisons of three type of fibers were done and the best fiber was the PDMS/DVB (65mum) fiber. The volatiles compounds identified consist of 3-methyl butanal, pentanal, methyl pyrazine, 2,5-dimethyl pyrazine, 2,6 dimethylpyrazine, ethyl pyrazine, benzaldehyde, limonene, linal ool, benzeneethanol, trimethylpyrazine, tetramethylpyrazine, benzeneacetaldehyde, nonanal, and 5-methyl-2-hexenal. All the compounds had been previously reported in cocoa volatiles.
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