Separation of Y-chromosome bearing bull’s spermatozoa using an albumin gradient technique
2011
Jasmi, Y. | Tan, Y.J. | Musaddin, K. | Somarny, W.W.M.Z. | Mat Tasol, S. | Johari, J.A.
This study was conducted to separate the X- and Y-chromosome bearing spermatozoa based on an albumin gradient technique. A modified but simple procedure was performed to minimize pressure on the spermatozoa. Two treatments consisting of two-phase albumin gradients which were 12.5% over 20% (Treatment 1), and 15% over 20% (Treatment 2) bovine serum albumin (BSA) were carried out to separate the X- and Y-chromosome bearing bull’s spermatozoa at room temperature. Subsequent analysis by FISH using a single labeled TSPY probe (specific for detection of Y-chromosome) showed that the albumin gradient technique was not effective (P>0.05) to separate the X- and Y- chromosome bearing spermatozoa. The percentage of Y-chromosome bearing spermatozoa recovered following separation by Treatment 2 was higher but not significant (P>0.05) compared to Treatment 1.
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