Optimization of β-galactosidase production from Penicilliumsp. for synthesis of galactooligosaccharides
2017
Almeida, M. M. | Cabral, P. S. | Chiquetto, N. C. | Bassetto, R. Z. | Silveira, M. H. L.
Enzymes are of great importance and have been widely used for the synthesis of prebiotics. In this study, process conditions of hydrolysis of lactose (pH, temperature and substrate concentration) were optimized through a surface response methodology to maximize the production of β-galactosidase (β-gal). A quadratic mathematical model was obtained and the optimum conditions was predicted to be at pH 5.0 and 60°C which corresponded to 1.8 U mg-¹ of lactase activity.The optimized broth was applied for synthesize galactooligosaccharides (GOS) in the presence of 25 or 35% (w/v) lactose source (whey).It was found that 25% whey gave higher lactose conversion.
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