Comparison of phytochemical and antioxidant properties of extracts from flaxseed (Linum usitatissimum) using different solvents
2018
Alachaher, F. Z. | Dali, S. | Dida, N. | Krouf, D.
The aim of the present study was to evaluate the phytochemical constituents and free radicals scavenging activities of flaxseed (Linum usitatissimum) solvents extract. For phytochemical analysis of extracts, preliminary screening methods of phytoconstitutents were followed in different solvents. In addition, total phenolic and flavonoids compounds were measured. In vitro antioxidant activity was determined by 2,2-diphenyl-2-picryl-hydrazyl(DPPH), superoxide radical scavenging activity and ferric reducing power tests. Phytochemical analysis provides evidence that both solvents extract of Linum usitatissimum contains an important amounts of bioactive compounds. Furthermore, using methanolic and butanolic were able to extract a higher amounts of total phenolic compounds (47.01±5.40 and 43.33±2.77 µg gallic acid equivalents/g of extract, respectively) and flavonoids (30.89±0.09 and 29.55±0.15 μg Quercetin equivalents/g of extract), respectively). In vitro antioxidant activities of petroleum ether, benzene, ethyl acetate, methanolic and butanolic extracts of flaxseed were evaluated. Butanolic extract exhibited a higher DPPH radical scavenging activity, whereas petroleum ether and ethyl acetate extracts showed an important hydrogen peroxides scavenging activity. Also, an increase of ferric reducing antioxidant power (FRAP) was noted with methanolic extract.The results of the current study showed that flaxseed contain important amount of phenolic compounds and higher antioxidant activities rendering them as a potential source for development of nutraceuticals.
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