Diagnostics of main bacterial agents of porcine respiratory diseases complex (PRDC) using PCR detection of Mycoplasma hyopneumoniae
Holko, I.(Statni Veterinarni Ustav, Prague (Czech Republic))E-mail:ivan.holko@atlas.cz | Urbanova, J.(Statny Veterinarny Ustav, Dolny Kubin (Slovak Republic)) | Holkova, T.(Statni Veterinarni Ustav, Prague (Czech Republic)) | Kmet, V.(Slovenska Akademia Vied, Kosice (Slovak Republic). Ustav Fyziologie Hospodarskych Zvierat)
The main goal of our work was to analyse the incidence of porcine respiratory disease complex (PRDC) regarding bacterial agents in the territory of northern districts of Slovakia. Mycoplasma hyopneumoniae and other secondary bacterial causative pathogens of PRDC comprised 75.2% of all cases (98) with clinical signs of respiratory infections that we examined in the course of one year. The detection of M. hyopneumoniae which is, like the whole rank of mycoplasmas, very difficult to cultivate was solved by using the PCR method with the direct isolation of M. hyopneumoniae from lung tissue. Antibiotic sensitivity testing of Pasteurella multocida and Actinobacillus pleuropneumoniae led to the conclusion that enrofloxacin is the most effective antibiotic in the therapy of PRDC in northern Slovakia.
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