Characterization of protoplast fusant between killer yeast and alcohol-fermenting yeast
1990
Chung, K.T. | Bang, K.W. | Chung, Y.J. (Kyungpook National Univ., Taegu (Korea Republic). Coll. of Agriculture) | Kim, J.K. (Keimyung Junior Coll., Taegu (Korea Republic). Dept. of Food and Nutrition) | Song, H.I. (Taegu Technical Junior Coll., Taegu (Korea Republic). Dept. of Food Technology)
Cell volume and DNA contents of the fusants were similar to those of parents. Genetic stability of the fusants was increased when they were cultured on minimal medium (MM) rather than on complete medium (CM), and the fusants were stabilized by subculturing 7 generations each 7 day on MM agar. The finally selected fusants after being cultured for 6 months on CM were stable without segregation. The fusants could also form nuclein and ascospores, and show red and pink colors by the test of TTC colorization. Assimilability and fermentability of carbon sources of the fusants were similar to those of parents. The tolerance of KCl, NaCl, sodium propionate and cycloheximide showed the traits of one strain of parents. When the fusants were cultured for 72 hr and 60 hr in the medium containing 20 % glucose and sucrose, respectively, the yield of ethanol for FWKS 260 was reached to 9.6 v/v % and 9.8 v/v %, respectively. The sensitive strain Kyokai 7 was found to be killed entirely after cultivation of 48 hr by the killer toxin from the fusants. The recipient S 29 and Kyokai 7 were found to have neither L nor M dsRNA plasmid. However, K 52 and fusants had both L and M dsRNA plasmid of 4.7 kb and 2.5 kb, respectively. The curants treated by heat and cycloheximide did not contain M dsRNA plasmid, but had large amounts of L dsRNA plasmid of those of killer yeasts
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