Isolation and optimization of cultivating conditions of alkalophilic strains for biodegradation of azo dye
1999
Kim, J.M. (Taekyeung College, Kyeungsan (Korea Republic). Department of Environmental Industry) | Chung, H.C. | Kwon, O.J. (Taekyeung College, Kyeungsan (Korea Republic). Department of Hotel Baking Technology)
In order to treat of alkaline dye-processing wastewater, alkalophilic strains biodegrading azo dye, Acid red 1, is isolated from natural system, and optimal culture conditions are examined using response surface analysis, statistical analysis system program. 15 different species which grow in alkaline culture media are isolated from the effluent and river soil discharged from wastesater treatment plant in dye industrial complex. One strain which has the best decolorization efficiency is chosen, and named as AR-1. The result of the examination of carbon, nitrogen and phosphorus sources which have influence on growth and decolorization reveals that optimum carbon, nitrogen and phosphorus sources are 1.0% fructose, 1.0% polypeptone, 1.0% yeast extract and 0.5% K2HPO4, respectively. In order to optimize of biodegradation conditions of dye by response surface analysis, the characteristics of decolorization and cell growth according to culture temperature and time are monitered. The result shows that the one is optimum 34.77 degrees centigrade for 12.97 hours; the other at 34.73 degrees centigrade for 12.96 hours. While, optimal conditions of culture that satisfy both cell growth and decolorization are the temperatures from 32.86 degrees centigrade to 36.36 degrees centigrade and the period of 10.96 to 15.75 hours, respectively.
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