Effect of inorganic salts and various bioreactors on the production of clavulanic acid
1999
Kim, I.C. | Kim, S.W. (Korea University, Seoul (Korea Republic). Department of Chemical Engineering)
For the efficient production of clavulanic acid, a mutant strain Streptomyces clavuligerus KK was selected from Streptomyces clavuligerus ATCC 27064 through mutation with NTG. S. clavuligerus ATCC 27064 produced about 200 mg/L of clavulanic acid when the medium was composed of 1%(v/v) glycerol, 1.5%(w/v) soybean flour, 0.1%(w/v) KH2PO4, 0.2%(v/v) soybean oil. A selected mutant, S. clavuligerus KK, produced about 1150mg/L of clavulanic acid in the same medium. After the addition of MgSO4 to the basal medium, S. clavuligerus KK produced about 1550 mg/L of clavulanic acid, which shows about 1.3 times higher than that produced in the basal medium. In order to select the proper bioreactor for the production of clavulanic acid, a batch culture was performed in an airlift, a bubble column and a stirred tank bioreactors. In an airlift bioreactor, about 1350 mg/L of clavulanic acid was produced, in a bubble column bioreactor, about 1550 mg/L, in a stirred tank bioreactor, about 2200 mg/L, respectively. The production of clavulanic acid in stirred tank bioreactor was about 50% higher than that by an airlift and a bubble column bioreactors. According to this result, the stirred tank bioreactor was selected as a proper bioreactor.
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