Isolation of an acid-labile gene from the seaweed Porphyra yezoensis tissue
1999
Jin, H.J. | Park, S.M. | Kim, L.G. | Kong, I.S. | Hong, Y.K. (Pukyong National University, Pusan (Korea Republic). Department of Biotechnology) | Jin, D.H. (Kangnung National University, Kangnung (Korea Republic). Faculty of Marine Bioscience and Technology)
The genetic response of aquaculturable weaweed Porphyra yezoensis tissue by acid shock have been compared using differential desplay technique. The tissue has been challenged in seawater containing 0.05% hydrogen chloride(pH 3.0) for 5 min, then rehabilitated in normal seawater for 10 min, 30 min, 60 min and 4 hrs, respectively. Total RNA was extracted by LiCl-guanidinium method. The cDNA was synthesized by reverse transcription with random hexamers and amplified by PCR with arbitrary primers. The genetic fragment disappeared by acid shock was selectively isolated from agarose gel and sequenced with a DNA auto sequencer. One of the acid-labile gene(605 bp) was identified as a dethiobiotin synthetase gene according to sequence alignment analysis by the MCBI BLAST search program
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