Selection of Effective Fungicides Against Xylogone sphaerospora, a Fungal Pathogen of Cultivated Mushroom, Ganoderma lucidum

A fungal disease of the cultivated mushroom, Ganoderma lucidum, caused by Xylogone sphaerospora was epidemic throughout all cultivaton areas in Korea which caused a lot of yield losses in the mushroom production. For controlling the disease, the screening of effcetive fungidides against the pathogenic fungus were conducted. Thirty seven commercially available fungicides were tested for their inhibitory activities on potato dextrose agar media supplimented with these fungicides at various concentrations. Twenty one fungicides significantly inhibited mycelial growth of the pathogen, Xylogone sphaerospora, but 16 fungicides had no inhibitory effect. Among these 21 fungicides, 17 fungicides also inhibited mycelial growth of Ganoderma lucidum as well, but imazalil, procymidone, triforine, and vinclozolin had no inhibitory effects. However, vinclozolin showed no inhibitory effect on mycelial growth of the mushroom even at the concentration of 50 micro g/ml. According to the result of in vitro bioassay, vinclozolin was selected for further in vivo tests to investigate the preventive effects on the pathogen. Logs for Ganoderma lucidum cultivation were dipped into the 500 micro g/ml vincolzolin solution for 2 hours, and then the pathogen was inoculated. After two month-cultivation of the mushroom, over 90% of logs treated with vinclozolin without pathogen inoculation produced fruiting bodies. However, fruiting bodies were not produced from the logs inoculated with the pathogen, but not treated with vinclozolin. Fifty seves percent of logs which were pre-treated with vinclozolin and then inoculated with the pathogen produced fruiting bodies. Based on the results, vinclozolin is effective for the control of yellow disease of the Ganoderma lucidum caused by Xylogone sphaerospora.