Screening and Characterization of Probiotic Strains for Prevention of Bacterial Fish Diseases
2003
Yang, B.G. | Jun, Y.J. | Heo, M.S. (Cheju National University, Jeju, Republic of Korea)
The purpose of the present study was to screen the effective of lactic acid bacteria as probiotics, which are able to protect aquacultural fish pathogenic bacteria, and investigate their characterization. Twenty strains of lactic acid bacteria were isolated from fish intestine, fermented fish foods and kimchis. These bacteria were screened for antagonistic activity against fish pathogenic bacteria. Seven tested LAB strains were able to inhibit the fish pathogenic bacteria, including Vibrio anguillarum, Edwardsiella tarda, and streptococcus sp.. Of the probiotic candidates, BK19 strain isolated from fermented pollack viscera indicated the a largest inhibition activity. Moreover, this strain showed a resistance over low pH and antibiotic agents. Therefore this probiotics candidate BK19 was was finally selected and identified as a probiotics strain. This particular probiotics bacteria was identified as Lactobacillus sakei BK19 by biochemical characteristics and 16S rRNA PCR amplification
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