Effects of Growth Factors and Hexoses on In Vitro Development in Porcine embryos
2003
Yoon, S.Y. | Lee, S.Y. | Cheong, H.T. | Yang, B.K. | Kim, J.I. | Park, C.K. (Kangwon National University, Chuncheon, Republic of Korea)
The aim of this study was to investigate the effects of different hexoses of medium with IGF-I and /or EGF on in vitro development of porcine embryos . In first experiment, when the embryos were cultured in medium with concentrations of 5ng/ml IGF-I and 10ng/ml EGF, the morula and blastocyst rates were higher than in another culture conditions. In second experiment the effect of IGF-I in mediu, with different hexoses during the embryos culture was examined. The higher cleavage rates were obtained in medium with glucose and IGF-I . However, the higher proportion of embryos developed to morula and blastocyst stages in medium with IGF-I were obtained than in medium without IGF-I in medium with glocose, mannose and galactose. In third experiment, the effect of EGF on in vitro development of porcine embryos in medium with different hexoses were examined.
Show more [+] Less [-]AGROVOC Keywords
Bibliographic information
This bibliographic record has been provided by Korea Agricultural Science Digital Library