Viability and Acrosomal Status Changes Following Post-thawing Caninbe Sopermatoza
2003
Lee, Y.R. | Lee, S.L. | Kang, T.Y. | Choi, S.Y. (Gyeongsang National University, Chinju, Republic of Korea)
This study was carried out to evaluate the effect of different freezing and thawing rates on the viability, mortility and acrosomal changes of frozen canine spermatozoa. The ejaculated semen was extended with Tris-egg yolk buffer containing 8% glycerol and equilibrated for 60 min after cooled to 4¡É for 58 min. The straws were cryopreserved gradually by slow-cooling at different distance from the liquid nitrogen to achieve temperature rate of 3,8.9 and 19¡É/min. Thawing of the straws was performed in a water bath for 2 min ar 37¡É and 55¡É, respectively
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