The Studies on Pregnancy after Transfer to Recipient with Blastocyst Derived In Vitro in Korean Cattle
2003
Kim, S.S. | Choi, S.H. | Kim, J.M. | Lee, J.H. | Kim, J.Y. | Park, H.D. (Chungbuk National University, Chungju. Republic of Korea)
These studies were carried out to establish an effective in vitro embryo transfer methods by analyzing several factors. The base media were TCM-199 solution for in vitro maturation of bovine follicular oocytes and Fer-TALP solution for in vitro fertilization and CR1aa medium for in vitro culture. IVC used the fertilized oocytes of 24-hr culture after IVF. Embryos were cultured in drop-culture that contained 25 embryos per 10mm. Blastocysts cultured for 7 to 9 in vitro were transferred to recipients.
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