Effects of Cold Shock Pretreatment and Carbohydrate Sources on Anther Culture of Rice
2003
Lee, K.W. | Won, Y.J. | Ko, J.M. | Park, H.M. | Cho, J.H. | Oh, B.K. | Yang, S.J. | Kim, S.C. | Nam, M.H. (National Yeongnam Agricultural Experiment Station, RDA, Milyang, Republic of Korea)
In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes;e.g. tongil and indica , is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2% to 12.5% maltose medium compared to the medium supplement rice IR50
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