Expression of the Promoter for the Maltogenic Amylase Gene in Bacillus subtilis 168
Kim, D.Y.;Cha, C.H.;Oh, W.S.;Yoon, Y.J.;Kim, J.W.(University of Incheon, Incheon, Republic of Korea)E-mail:kjw5864@incheon.ac.kr
An additional amylase, besides the typical α-amylase, was detected for the first time in the cytoplasm of B. subtilis SUH4-2, an isolate from Korean soil. The corresponding gene (bbmA) encoded a maltogenic amylase (MAase) and its sequence was almost identical to the yvdF gene of B. subtilis 168, whose function was unknown. Southern blot analysis using bbmA as the probe indicated that this gene was ubiquitous among various B. subtilis strains. In an effort to understand the physiological function of the bbmA gene in B. subtilis, the expression pattern of the gene was monitored by measuring the β-galactosidase activity produced from the bbmA promoter fused to the amino terminus of the lacZ structural gene, which was then integrated into the amyE locus on the B. subtilis 168 chromosome.
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