Effect of Myo-Inositol on In Vitro Maturation of Porcine Oocytes
Cho, I.S.;Han, H.W.;Lee, S.M.;Park, H.Y.;Jeoung, Y.H.;Moon, S.J.;Kang, M.J.(Chonnam National University, Gwangju, Republic of Korea)E-mail:[email protected] | Kang, S.Y.(National Institute of Subtropical Agriculture, RDA, Jeju, Republic of Korea)
This study was carried out to assess whether the addition of myo-inositol to maturation medium could improve porcine oocyte maturation in vitro. Oocytes were cultured for the first 22 h in Witten's medium containing 10IU/㎖ PMSG, 10 IU/㎖ HCG supplemented with or without myo-inositol. Subsequently, they were cultured for additional 22 h in Witten's medium without hormone supplemented with or without myo-inositol. When the porcine oocytes were cultured in maturation medium containing myo-inositol, the proportion of metaphase Ⅱ oocytes 44h after culture was higher in the myo-inositol group(P less than 0.05).
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