Effects of Different Media and Oxygen Concentrations on In Vitro Maturation and Development of Porcine Follicular Oocytes
Cheon, H.S.;Kim, J.H.;Lee, K.S.;Seo, K.W.(Chungnam National University, Daejeon, Republic of Korea)E-mail:[email protected] | Han, M.H.(National Livestock Research Institute, RDA, Suwon, Republic of Korea) | Park, B.K.(Kong-Ju University, Kongju, Republic of Korea)
The present study was carried out to examine the effect of four different media (NCSU (North Carolina State University)-23, PZM (Porcine Zygotes Medium)-3, PZM-4 and TCM (Tissue Culture Medium)-l99) and two oxygen concentrations (39 , 5% O₂, 5% CO₂ and 90% N₂, 5% CO₂ in air) on in vitro production of porcine IVM/IVF embryos. The results were summarized as follows: The rates of GVBD and nuclear maturations were not significantly different (p0.05) for 44 hours of culture with four media in two oxygen concentrations. The rates of polyspermy, penetrated sperm(s) and male and female prouclei formation were not significantly different (p greater than 0.05). among four media in two oxygen concentrations.
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