Sow Transfer of Cultured Freezing Embryos by Open Pulled Straw(OPS) Methods : Preliminary Results
Kim, I.D.;Ahn, M.H.;Seok, H.B.(Dankook University, Cheonan, Republic of Korea)E-mail:[email protected] | Hur, T.Y.(National Livestock Research Institute, Seungwhan, Republic of Korea) | Hong, M.P.(Moonseong Pig Farm, Pyeongtaek, Republic of Korea)
The aims of this study are 1) to test oocytes and embryos collected from in-vitro to achieving the valuable protocol by culturing, vitrifying and thawing of oocytes/embryos, and 2) to transfer them to recipient, and finally have resulted in pregnancies from recipient females after surgical or nonsurgical transfer. In vitro maturation and fertilization were performed according to Funahashi et al (1994). Glucose-free NCSU 23 supplemented with 5 mM sodium pyruvate, 0.5 mM sodium lactate and 4 mg/ml bovine serum albumin for 2 days at 39℃, and 10% fetal bovine serum albumin was added to the culture medium thereafter.
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