Antimicrobial Effect of Chitosan and Chitooligosaccharides against Bacterial Diseases of Cultured Flounder
Yang, B.G.;Lee, J.H.;Kim, S.H.;Jeon, Y.J.(Cheju National University, Jeju, Republic of Korea)E-mail:youjinj@cheju.ac.kr
Antimicrobial effect of chitosan and its oligosaccharides was examined on Vibrios sp., Edwardsiella tarda and Streptococcus sp., which are major pathogenic bacteria inducing bacterial diseases of acquacultured flounder. Chitosan oligosaccharides (COS ) were produced by enzymatic hydrolysis of chitosan in an ultrafiltration mombrane bioreactor system which was established with three membranes with different molecular weight cut-off (MWCO) 1,000, 5,000 and 10,000, and fractionated into three kinds of COS, based on their molecular weight sizes. The three kinds of COS were as follows: relatively high molecular weight COS [HMW-COS, molecular weight distribution of 7,000 to 24,000 Da], medium molecular weight COS [MMW-COS, 1,500 to 6,000 Da], and low molecular weight COS [LMW-COS, 1,000 to 1,500 Da].
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