Inhibition of Lipid Autoxidation by the Extract of the Submerged-liquid Culture of Mushrooms in the Medium Containing Mulberry Tree Powders
Kim, S.J.;Hyung, S.W.;Kim, M.S.;Ha, Y.L.(Gyeongsang National University, Jinju, Republic of Korea)E-mail:[email protected] | Lim, D.K.(Busan Regional KFDA, Busan, Republic of Korea) | Kim, J.O.;Kim, M.N.(HK Biotech. Co., Ltd, Jinju, Republic of Korea) | Lee, K.K.(Samsung Everland, Yongin, Republic of Korea)
Effect of mulberry tree powders on the antioxidant activity of submerged -liquid culture of mushrooms was investigated. Agaricus blazei (AB), Hericicum erinacium (HE), Pleurotus ostreatus (PO), Phellinus linteu (PL) and Paecilomyces japonicus (PJ) were cultured in a shaking incubator (200 rpm, 25℃) for 7 days in culture media: 1) basal medium (BM) and 2) BM+1% mulberry tree powders (BMM). Hot water extracts from the submerged-liquid cultures of mushrooms and BMM were freeze-dried for the measurement of antioxidant activity, of which reaction mixture (25 mL: 10 mL of 0.2 M sodium phosphate buffer, pH 8.04.5 mL distilled waterand 10.5 mL ethanol) contained 275 μmol linoleic acid and one ㎎ test sample.
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