Cellular Iron Uptake from Aqueous Solutions depending on Reaction Conditions by genetically engineered Saccharomyces cerevisiae
Kim, S.J.;Chang, Y.J.;Park, C.U.;Jeong, Y.S.;Kim, K.S.(Chonbuk National University, Jeonju, Republic of Korea)E-mail:sukkim@chonbuk.ac.kr
Cellular iron uptake was performed in the yeast Saccharomyces cerevisiae that transformed with human ferritin H- and L-chain genes. The recombinant yeasts were enriched in YEP medium supplemented with 2% galactose for 3 days and the iron uptake was followed by incubating the cells with iron in 20 mM MOPS buffer (pH 6.5). The reactions were examined under different conditions including the iron compounds of Fe(Ⅱ) and Fe(Ⅲ), the concentration of iron, the concentration of cells and the reaction time.
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