The Application of RNA Transcript Conformation Polymorphism in Resolving Mixed Infection of PVY Isolates
Rosner, Arie;Maslenin, Ludmila(the Volcani Center, Bet Dagan, Israel)E-mail:rosnera@agri.gov.il
A method based on RNA-transcript conformation polymorphism (TCP) was tested for detection of two PVY isolation in a mixed infection. Differences in electrophoretic mobility of RNA transcripts copied from PCR products of each virus isolate enabled the distinction between the two virus isolates in a mixed infection. The identities of the RNA transcripts and hence of the infecting virus isolates were determined by annealing to reference oligonucleotides containing unique strain-specific sequences visualized by retardation of transcript mobility in gel.
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