Bioelectrocatalyzed Signal Amplification for Affinity Interactions at Chemically Modified Electrodes
Yoon, H.C.(Ajou University, Suwon, Republic of Korea)E-mail:hcyoon@ajou.ac.kr | Kim, H.S.(Korea Advanced Institute of Science and Technology, Daejeon, Republic of Korea)
A comparative study was performed to evaluate the signal amplification strategies in electrochemical affinity sensing, which included the direct electron transfer and diffusible-group mediated electron transfer between label enzymes that were specifically bound to target proteins and chemically modified electrode surfaces. As a platform surface for affinity recognition reactions, a double functionalized poly(amidoamine) dendrimer monolayer that was modified with ferrocene and biotin groups was constructed on a gold surface.
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