Cloning and Characterization of the Lactate Dehydrogenase Genes from Lactobacillus sp. RKY2
Lee, J.H.;Choi, M.H.;Park, J.Y.;Kang, H.K.;Ryu, H.W.;Sunwo, C.S.;Wee, Y.J.;Park, K.D.;Kim, D.M.(Chonnam National University, Gwangju, Republic of Korea)E-mail:dmkim@chonnam.ac.kr | Kim, D.W.(Kangnung National University, Kangnung, Republic of Korea)
Lactic acid is an environmentally benign organic acid that could be used as a raw material for biodegradable plastics if it can be inexpensively produced by fermentation. Two genes (ldhL and ldhD) encoding the L-(+) and D-(-) lactate dehydrogenases (L-LDH and D-LDH) were cloned from Lactobacillus sp., RKY2, which is a lactic acid hyper-producing bacterium isolated from Kimchi. Open reading frames of ldhL for and ldhD for the L and D-LDH genes were 962 and 998 bp, respectively.
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