Application of RAPD and SCAR Markers for Purity Testing of F₁ Hybrid Seed in Chili Pepper (Capsicum annuum)
Jang, I.O.(Seoul Women's University, Seoul, Republic of Korea) | Moon, J.H.;Yoon, J.B.;Yoo, J.H.;Yang, T.J.;Kim, Y.J.;Park, H.G(Seoul National University, Seoul, Republic of Korea)E-mail:hgpark@plaza.snu.ac.kr
A simpler and better method for purity testing of hybrid pepper seed was developed. The simplest method for extracting genomic DNA, the NaOH method, was chosen. Two RAPD markers identifying male and female parents were also developed, and the PCR products of male- and female-specific RAPD markers were cloned and sequenced. From these sequences, new longer primers were constructed for conversion into SCAR markers. In blind tests the RAPD and SCAR markers were able to reliably detect contaminating exotic seeds.
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