Induction of Hariy Root and Bioreactor Culture of Lycium chinense
Bae, K.H.(Microplants Co., Ltd., Jeonju, Republic of Korea) | Kim, Y.S.(Chung-Ang University, Anseong, Republic of Korea) | Jeong, J.H.(Korea Forest Research Institute, Suwon, Republic of Korea) | Kim, Y.S.(Namdo Provincial College of Jeonnam, Changhung, Republic of Korea) | Choi, Y.E.(Kangwon National University, Chuncheon, Republic of Korea) | Yoon, E.S.(Kong-ju National University, Gongju, Republic of Korea)E-mail:[email protected]
This article was conducted to induce the transgenic hairy roots and determine the effect of culture conditions on optimum growth of hairy roots by Agrobactrium rhizogenes strain, 15834 in Lycium chinense Miller. Hairy roots of L. chinense Miller. Were induced from leaf segments by co-cultivation with A. rhizogenes. When the hairy roots were cultured in various MS medium strength and sucrose concentrations, the highest growth of hairy roots was observed in half-strength MS medial supplemented with 3% sucrose, respectively.
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