Engineering Recombinant Streptomyces coelicolor Malate Synthase with Improved Thermal Properties by Directed Mutagenesis
2004
Koh, Rosita (National University of Singapore, Singapore) | Goh, Liuh Ling (National University of Singapore, Singapore) | Sim, T.S. (National University of Singapore, Singapore), E-mail: micsimts@nus.edu.sg
Streptomyces thermovulgans malate synthase (stMS) is known to be more thermostable and thermoactive than S. coelicolor malate synthase (scMS). Therefore, based on the amino acid sequence of stMS, 3 scMS mutants, namely P186R, T8PL9P, and T8PL9PP186R, were created by site-directed mutagenesis in an attempt to engineer a more thermoactive and thermostable enzyme. An enzymatic analysis of the wild-type and mutant MS revealed that P186R and T8PL9PP186R were more thermoactive than the wild-type scMS and T8PL9P.
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