Growth Temperature-Dependent Conversion of De novo-Synthesized Unsaturated Fatty Acids into Polyhydroxyalkanoic Acid and Membrane Cyclopropane Fatty Acids in the Psychrotrophic Bacterium Pseudomonas fluorescens BM07
2004
Lee, H.J. (Gyeongsang National University, Chinju, Republic of Korea) | Rho, J.K. (Gyeongsang National University, Chinju, Republic of Korea) | Yoon, S.C. (Gyeongsang National University, Chinju, Republic of Korea), E-mail: scyoon@nongae.gsnu.ac.kr
A psychrotrophic bacterial strain, Pseudomonas fluorescens BM07, synthesized unsaturated fatty acids (UFA) from fructose in response to lowering of growth temperature, and incorporated them into both polyhydroxyalkanoic acid (PHA) and membrane lipid. The blocking of PHA synthesis by adding 5 mM 2-bromooctanoic acid to the growth medium, containing 70 mM fructose, was found to be a useful means to profile the composition of membrane lipid by gas chromatography. As the growth temperature changed from 35 to 5℃, the total content of two UFA, 3-hydroxy-cis-5-dodecenoic acid (C∧12:1) and 3-hydroxy-cis-7-tetradecenoic acid (C∧14:1), in PHA increased from 31 to 44 mol%.
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