Study on Effectiveness of Vitrification using Electron Microscope Grid in Mice Embryo Cryopreservation
2004
Lee, E.J. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Song, H.B. (Daegu University, Gyeongsan, Republic of Korea) | Park, C.S. (Chungnam National University, Daejeon, Republic of Korea) | Moon, O.S. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Choi, Y.K. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Kim, H.C. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea), E-mail: hckim@kribb.re.kr
This present study was conducted to examine the effectiveness of vitrification by electron microscope (EM) grid in cryopreservation of mouse 2cell embryos. A total of 2,744 mouse 2-cell embryos were used, and the rate of survival and development in vitro after freezing-thawing by three different cryopreservation methods were comparatively examined. In the experiment of only exposre on freezing and thawing solution, the survival rates of 2-cell mice embryos were not significant difference between vitrification method (VM) Ⅰand Ⅱ (90.4% and 93.3%, respectively), and also, the rates of development into blasocyst were not significant difference between two groups (76.4% and 78.0%, respectively) after 72h in culture in vitro.
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