Nitrate Uptake in the Halotolerant Cyanobacterium Aphanothece halophytica is energy-dependent driven by ΔpH
2005
Incharoensakdi, Aran (Chulalongkorn University, Bangkok, Thailand), E-mail: iaran@sc.chula.ac.th | Laloknam, Surasak (Chulalongkorn University, Bangkok, Thailand)
The energetics of nitrate uptake by intact cells of the halotolerant cyanobacterium Aphanothece halophytica were investigated. Nitrate uptake was inhibited by various protonophores suggesting the coupling of nitrate uptake to the proton motive force. An artificially-generated pH gradient across the membrane (ΔpH) caused an increase of nitrate uptake. In contrast, the suppression of ΔpH resulted in a decrease of nitrate uptake. The increase of external pH also resulted in an enhancement of nitrate uptake. The generation of the electrical potential across the membrane (Δψ) resulted in no elevation of the rate of nitrate uptake. On the other hand, the valinomycin-mediated dissipation of Δψ caused no depression of the rate of nitrate uptake.
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