Production of Transgenic Maize (Zea mays L.) Using Agrobacterium tumefaciens-Mediated Transformation
2005
Cho, M.A. (Eugentech Inc., Daejeon, Republic of Korea) | Kim, J.S. (Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea) | Park, K.J. (Maize Experiment Station, Gangwon-do Provincial ARES, Hongcheon, Republic of Korea) | Liu, J.R. (Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea) | Clemente, Tom (University of Nebraska-Lincoln, Lincoln, NE, USA) | Choi, P.S. (Nambu University, Kwangju, Republic of Korea), E-mail: cps6546@nambu.ac.kr | Park, Y.O. (Eugentech Inc., Daejeon, Republic of Korea) | Min, H.K. (Maize Experiment Station, Gangwon-do Provincial ARES, Hongcheon, Republic of Korea)
Agrobacterium tumefaciens-mediated immature embryo transformation was use to produce transgenic maize. Immature embryo of Hi Ⅱ genotype were co-cultivated with strains Agrobacterium tumefaciens (C58C1) containing the binary vectors (pPTN290) carrying with Ubiquitin promoter-GUS gene as reporter gene and NOS promoter-nptⅡ gene conferring resistance to paromomycin as selective agent. Seven embryogenic callus lines transformed showed the resistance in paromomycin antibiotics.
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