Capillary Electrophoretic Method for the Determination of (+)-Catechin, (-)-Epicatechin in Grape Seed Ethanol Extract
2005
Choi, O.K. (Hankyong National University, Ansung, Republic of Korea), E-mail: eoflow@hanmail.net | Chung, Y.S. (Hankyong National University, Ansung, Republic of Korea) | Chung, H.Y. (Hankyong National University, Ansung, Republic of Korea)
Capillary electrophoresis (CE) method was developed to determine (+)-catechin and (-)-epicatechin contents in grape seed ethanol extract. CE separation was achieved using 100 mM phosphate and borate buffer at pH 6.0 as background electrolyte and fused silica capillary with 50 microns i.d. x 375 microns O.D. (effective length 20.0 cm) maintained at 25℃. The applied voltage was 10 kV, and detection was performed by DAD at 210 nm. Two catechins were well separated within 6 min with repeatability of less than 0.8% RSD for migration time and less than 2.0% RSD for peak area, and correlation coefficients higher than 0.994 were obtained from 58.0 to 174.0 mg/L with detection limit of 0.035 mg/L.
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