Simplified Procedure for Detection of Poliovirus and Norovirus in Oysters
2005
Ha, S.H. (Dongguk University, Gyeongju, Republic of Korea) | Woo, G.J. (Korea Food and Drug Administration, Seoul, Republic of Korea) | Kwak, H.S. (Korea Food and Drug Administration, Seoul, Republic of Korea) | Hwang, I.G. (Korea Food and Drug Administration, Seoul, Republic of Korea) | Choi, W.S. (Dongguk University, Gyeongju, Republic of Korea), E-mail: weonsang@dongguk.ac.kr
Simplified procedure was developed for concentrating and detecting poliovirus and norovirus in oysters. Viruses were seeded into oyster tissue homogenates and concentrated through polyethylene glycol (PEG) precipitation, chloroform or Freon extraction, with additional PEG precipitation. Amount of viruses was evaluated using poliovirus plaque assay. Virus recovery during concentration procedure was approximately 16.4-26.0%. For detection viral RNAs in oysters were examined using one-step RT-PCR after extraction with Trizol.
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