Isolation, Characterization, and Investigation of Surface and Hemolytic Activities of a Lipopeptide Biosurfactant Produced by Bacillus subtilis ATCC 6633
2005
Dehghan-Noudeh, Gholamreza (Kerman University of Medical Sciences, Kerman, Iran), E-mail: grdehghan@yahoo.com | Housaindokht, Mohammadreza (Ferdowsi University, Mashhad, Iran) | Fazly Bazzaz, Bibi Sedigeh (Mashhad University of Medical Sciences, Mashhad, Iran)
Bacillus subtilis ATCC 6633 was grown in BHIB medium supplemented with Mn²+ for 96 h at 37℃ in a shaker incubator. After removing the microbial biomass, a lipopeptide biosurfactant was extracted from the supernatant. Its structure was established by chemical and spectroscopy methods. The structure was confirmed by physical properties, such as Hydrophile-Lipophile Balance (HLB), surface activity and erythrocyte hemolytic capacity. The critical micelle concentration (cmc) and erythrocyte hemolytic capacity of the biosurfactant were compared to those of surfactants such as SDS, BC (benzalkonium chloride), TTAB (tetradecyltrimethylammonium bromide) and HTAB (hexadecyltrimethylammonium bromide).
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