Retinol Supplemented to a Maturation Medium Stimulated In Vitro Development of Porcine Oocytes after In Vitro Fertilization
2006
Kim, Y.S. (Jinju National University, Jinju, Republic of Korea) | Song, S.H. (Jinju National University, Jinju, Republic of Korea) | Cho, S.K. (Jinju National University, Jinju, Republic of Korea) | Kwack, D.O. (Jinju National University, Jinju, Republic of Korea) | Kim, C.W. (Jinju National University, Jinju, Republic of Korea) | Park, C.S. (Jinju National University, Jinju, Republic of Korea) | Chung, K.H. (Jinju National University, Jinju, Republic of Korea), E-mail: kchung@jinju.ac.kr
The objective of this study was to investigative the effects of retinol supplement of IVM and/or IVC medium on maturation, fertilization and development of pig oocytes. North Carolina State University (NCSU) 23 medium containing porcine follicular fluid (pFF) was used as base medium. Each 1 uM, 5 uM and 10 uM concentration of retinol was supplemented to IVM and /or IVC medium. When the retinol was supplemented to maturation medium, the maturation rates were not different (p greater than 0.05) among treatment groups (66.7±6.0~69.2±5.3%), but the developmental rate to blastocyst stage was higher (p less than 0.05) in 5 μM group (20.4±2.6%) than in 0 uM (13.6±2.1%) and 10 uM groups (9.7±1.7%).
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