Effects of Donor Cells and Estrus Synchronization on the Production of Cloned Korean Native Goat
2006
Park, H.S. (Jinju National University, Jinju, Republic of Korea), E-mail: [email protected] | Kim, T.S. (Jinju National University, Jinju, Republic of Korea) | Jung, S.Y. (Jinju National University, Jinju, Republic of Korea) | Park, J.K. (Gyeongnam Province Advanced Swine Research Institute, Sanchung, Republic of Korea) | Lee, J.S. (Jinju National University, Jinju, Republic of Korea) | Jung, J.Y. (Jinju National University, Jinju, Republic of Korea)
The objective of this study was to examine the effect of donor cell types, the source of recipient oocytes and estrous synchronization on pregnancy and delivery rates of somatic cell nuclear transfer (SCNT) embryos in Korean native goats. Recipient oocytes were surgically collected after superovulation. Ear cells and fetal fibroblasts were collected and cultured in serum-starvation condition (TCM-199 +0.5% FBS) for cell confluence. The zonae pellucidae of in vivo-and in vitro-matured oocytes were partially drilled using a laser system.
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