Identification and Growth Activity to Bifidobacterium spp. of Locust Bean Gum Hydrolysates by Trichoderma harzianum β-mannanase
2005
Kim, Y.J. (Kyungwon University, Seongnam, Republic of Korea) | Park, G.G. (Kyungwon University, Seongnam, Republic of Korea), E-mail: ggpark@kyungwon.ac.kr
This study was performed to elucidate substrate specificity to the locust bean gum galactomannan by Trichoderma harzianum β-mannanase. The medium composition for enzyme production were determined 3% cellulose, 3% corn steep liquor, 1% KH₂PO₄, 0.2% (NH₄)₂SO₄, and incubated for 115 hr at 28℃. The β-mannanase exhibited maximum activity at pH 4.5 and 60℃. Locust bean gum galactomannan was hydrolyzed by the β-mannanase, and then hydrolysates separated by activated carbon column chromatography.
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